报告题目：Membrane Curvature, a New Code to Hack Live Cell at Nanoscale

报告人：新加坡南洋理工大学 赵文婷 副教授

报告时间：2018年6月7日（周四）上午9：00

报告地点：学院会议室（子良A220）

邀请人：新能源材料与技术研究所  陶新永  教授

报告人简介：

Dr. Wenting Zhao joined the School of Chemical and Biomedical Engineering at Nanyang Technological University in August 2017. She graduated from Zhejiang University in China with a B. Eng in Bioengineering and later from Hong Kong University of Science and Technology with a Ph.D in Bioengineering. After that, she did her postdoc training at Stanford University, working in both Prof. Yi Cui's group in Materials Science and Engineering and Prof. Bianxiao Cui's group in Chemistry. Her research interest is studying the interplay between biology and materials, with specific focuses on leveraging cutting-edge nanofabrication technologies to develop new methodologies and platforms for the manipulation of nanoscopic cellular features. Her research works have been published in Nature Nanotechnology, PNAS, Nano Letters, ACS Nano, Chemical Communications, etc.

1. Lou, H.Y.*; Zhao, W. T.*; (* equal contribution) Zeng, Y.; Cui, B. X. Accounts of Chemical Research, 2018. ASAP.

2. Nagy, N., de la Zerda, A., Kaber, G., Johnson, P., Y., Hu, K. H., Kratochvil, M. J., Yadava, K., Zhao, W. T., Cui, Y., Navarro, G., Annes, J. P., Wight, T. N., Heilshorn, S. C., Bollyky, P. L., Butte, M. J.Journal of Biological Chemistry, 2018, 293, (2), 567-578.

3. Zhang, R., Liu, C., Zhou, G., Sun, J., Liu, N., Hsu, P. C., Wang, H., Qiu, Y., Zhao, J., Wu, T., Zhao, W. T. Cui, Y. Nano Research, 2017, (DOI: 10.1007/s12274-017-1724-y).

4. Zhao, W. T.; Hanson, L.; Lou, H.Y.; Akamatsu, M.; Chowdary, P.; Santoro, F.; Marks, J.; Grassart, A.; Drubin, D. G., Cui, Y.; Cui, B. X. Nature Nanotechnology, 2017, 12, 750-756.

5. Lou, H.Y.*; Zhao, W. T.*; (* equal contribution) Hanson, L.; Zeng, C.; Cui, Y.; Cui, B. X. Langmuir, 2017, 33, (4), 1097-1104.

6. Hanson, L.; Zhao, W. T.; Lou, H.Y.; Lin, Z. L.; Lee, S.W.; Chowdary, P.; Cui, Y.; Cui, B. X. Nature Nanotechnology, 2015, 10, (6), 554-562.

7. Xie, X.*; Zhao, W. T.*; (* equal contribution) Lee, H. R.; Liu, C.; Ye, M.; Xie, W. J.; Cui, B. X.; Criddle, C. S.; Cui, Y. ACS Nano, 2014, 8, (12), 11958-11965.

8. Liu, N.; Lu, Z.; Zhao, J.; McDowell, M.; Lee, H. W.; Zhao, W. T.; Cui, Y. Nature Nanotechnology, 2014, 9, (3), 187-192.

9. Liu, C.; Xie, X.; Zhao, W. T.; Liu, N.; Maraccini, P. A.; Sassoubre, L. M.; Boehm, A. B.; Cui, Y. Nano Letters, 2013,13,(9),4288-4293.

报告内容简述：

Cellular membranes deform strikingly during many processes, e.g. endocytosis, morphogenesis, mitosis and vesicle trafficking. The molecular mechanism behind, however, remains unclear. Membrane deformations usually happen at subcellular scale, i.e., the scale of tens to hundreds of nanometers. A key challenge to study such delicate but dramatic processes is the lack of technologies to control or manipulate nanoscale membrane deformations in live cells. In this talk, I will introduce our efforts in developing a nanoplatform to generate pre-defined membrane deformations in live cells, and demonstrate the influence of on endocytosis, actin polymerization, and nuclear mechanics. On our platform, plasma membranes can be deformed to a wide range of curvature from +100 nm to -500 nm radius. The positively curved membranes serve as preferred hotspots for clathrin-mediated endocytosis (CME) and that the key CME proteins, clathrin and dynamin, show a strong preference to curvatures with a radius < 200 nm. Besides, actin polymerization could also be guided by the nanoscale curved membrane, as well as the deformation of the nucleus. Our study demonstrates a controlled manipulation of nanoscale membrane deformation in live cells, which shed light on a new angle to understand and engineer the complex and dynamic biological system.